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bkdR [2018-12-09 18:14:38]
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bkdR [2018-12-09 18:14:38]

transcriptional activator of the ptb-bcd-buk-lpdV-bkdAA-bkdAB-bkdB operon
locus
BSU24100
pI
6.24
mw
77.75 kDa
protein length
692 aa Sequence Blast
gene length
2076 bp Sequence Blast
function
regulation of branched-chain amino acid utilization,
product
transcriptional activator (for SigL-dependent promoter))
essential
no
synonyms
yqiR
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.2|Utilization of amino acids] → [category|SW 2.3.2.9|Utilization of branched-chain amino acids]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.5|Transcription factors/ other]

    • Gene

    Coordinates
    2,504,789 → 2,506,867

    Phenotypes of a mutant

  • The mutant is cold-sensitive [http://www.ncbi.nlm.nih.gov/sites/entrez/16585774 PubMed]

    • The protein

    Protein family

  • [SW|Transcription factors activating transcription at SigL-dependent promoters]

    • [SW|Domains]

  • [SW|PAS domain]

    • Expression and Regulation

    Operons

    genes
    [gene|4CEBD81F485DD0B660E297FFC34A0F5270652184|bkdR]
    description
    [Pubmed|12823818]

    regulatory mechanism

  • [protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY]: repression, [Pubmed|10094682], in [regulon|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY regulon]

    • regulation

  • repressed during growth in the presence of branched chain amino acids ([protein|search|CodY]) [Pubmed|10094682]
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    Biological materials

    Mutant

  • MGNA-C375 (yqiR::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2373 NBRP B. subtilis, Japan]
  • 1A913 ( ''bkdR''::''kan''), [Pubmed|16585774], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A913&Search=1A913 BGSC] and in [SW|Jörg Stülke]'s lab
  • BKE24100 (Δ[gene|4CEBD81F485DD0B660E297FFC34A0F5270652184|bkdR]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE24100 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCCCGATACCCCTTTGTA, downstream forward: _UP4_TAATTTGCAGAATAAACGCA
  • BKK24100 (Δ[gene|4CEBD81F485DD0B660E297FFC34A0F5270652184|bkdR]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK24100 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCCCGATACCCCTTTGTA, downstream forward: _UP4_TAATTTGCAGAATAAACGCA

    • References

  • 16585774,10094682,23123912,12823818,21906631